Mudasir Rashid

Postdoctoral researcher and Instructor · Department of Medicine, Gastroenterology Division,

Author

APID: 2854
ORCID: 0000-0001-9342-6243
Google Scholar: https://scholar.google.com/citations?user=EjdVSIcAAAAJ&hl=en
Location: Washington, DC, United States
Expertise: Epi-genetic Techniques: Bisulfite conversion method, Methylated DNA Immunoprecipitation (MeDIP), HAT and HDAC assay. • Molecular Biology Techniques: Droplet Digital PCR (ddPCR), Real time-PCR. • Cell Culture and Cancer Biology Techniques: Handling mammalian cell cultures, Transfection, Luciferase assay, Proliferation assay (MTTs and Clonogenic assay), Migration/ Invasion assay (Wound healing, Boyden Chamber). • Analytical and Immunological Techniques: SDS-PAGE, AUT-PAGE, Western-blotting, Co-immunoprecipitation (Co-IP), Immunofluorescence (IF), Immunohistochemistry (IHC) and Micrococcal Nuclease assay (MNase), Comet assay. • Microbiology: Competent cell preparation, transformation, screening and plating, cloning (primer designing), site-directed mutagenesis (SDM), and Protein purification (using combination of ion exchange, size exclusion, and affinity chromatography techniques). Knock-in point-mutation using CRISPR-Cas9 HDR based methods. • RNA seq analysis: RNA isolation, cDNA synthesis, library preparation, adapter ligation, and data analysis.
Areas of interest: Epigenetics, Microbiome, Cancer biology-Colorectal cancer and saffron
Member since: August 2023

Biography

I have extensive expertise in Cancer Biology, Epigenetics, Biotechnology, Microbiome. I hold a Master of Science (MS) in Biotechnology from the University of Bangalore, India, and a Doctor of Philosophy (PhD) in life science with a focus on Cancer Biology, specifically Gastric Adenocarcinoma.
Currently, I am working in Dr. Ashktorab's laboratory as a Postdoctoral Researcher in Department of Medicine, Gastroenterology Division, Howard University Hospital Cancer Center, Washington DC, USA.
During my doctoral studies, I focused on understanding the molecular profiles of histone H3 isoforms and variants in gastric adenocarcinoma and exploring novel pathways and potential therapeutic targets. At Howard University, I have conducted exome next-generation sequencing (NGS) on African American colorectal cancer (CRC) patients, leading to the identification of novel and deleterious MSH3 missense mutations, providing insights into their significance in AA-CRC pathogenesis.