Mr. Bhupendra kumar

Graduate Student · Department of Biotechnology

Helix BioGenesis Pvt. Ltd. A-52, Sector-2, Noida, Near_Gate_No._3, Noida_Sector-15_Metro_Station, Delhi, Uttar Pradesh 201301

Author

APID

9172

ORCID

https://orcid.org/0009-0004- 9528-1833

Location

Khurja, Uttar Pradesh, India

Expertise

•         Freezing & thawing of human cancer cell lines  •         RNA extraction from human cancer cell lines  •         cDNA synthesis  •         Polymerase Chain Reaction (PCR)  •         Induction of proliferation and differentiation in human cancer cell lines  •         Protein extraction from human cancer cell lines  •         SDS-PAGE and Coomassie blue staining  •         MTT Assay for proliferation/cytotoxicity  •         Restriction digestion  •         Ligation  •         DNA gel electrophoresis  •         DNA gel extraction  •         Preparation of competent cells  •         Transformation and plating on LBA plates  •         Plasmid DNA isolation

Areas of interest

Molecular Biology, Cancer Biology, Neuroscience, Cell Biology

Member since

January 2025

Publications

1. Programmed cell death