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Sworna kumari Chithiraivelu

Dr. Sworna kumari Chithiraivelu

PhD student · Department of animal biotechnology
Author
APID
9231
Location
Chennai, Tamilnadu, India
Expertise
Molecular biology
Areas of interest
Mab Production | DNA VACCINE -IBD Virus | Bioplastic -Microsphere Encapsulation |cloning and expression
Member since
January 2025

Biography

• Molecular Biology -DNA vaccine development, Cloning and expression of recombinant proteins, ELISA, Monoclonal antibody production-screening, Handled Bioreactor/ fermenter, Lyophilizers, Real Time PCR, microsphere preparation and in vitro controlled drug release, Latex agglutination test, Lateral flow assay, Electrophoresis, Immunoelectrophoresis
• Cell culture work- Sub culturing cell line like VERO, CHO, BHK and MDBK, Cell Viability, Invitro cytotoxicity assay, Lymphocyte Proliferation Assay, TCID, Cell Cycle analysis by BrDU, transfection, infection of viruses, Cryopreservation, and reviving
• Animal handled- Balb/c Mice, Chickens (SPF Eggs)
• Protein biochemistry -SDS PAGE Western Blotting, Protein purification, Dialyzing the protein
• Microbiology work-Isolation and Identification of Micro-organisms, Staining Techniques, Plant Biotechnology work (Genetic Modification and budding plants/plant products)
• Bioinformatics- Databases, Sequence alignment, Primer designing, sequence submission, Phylogenetic Tree construction, Insilico-PCR
• Embryo biotechnology- Oocyte collection, Invitro maturation of oocytes, Invitro fertilization, assessment, grading, Embryo biopsy collection using micromanipulator, ICSI, Sexing of embryos by PCR, Cryopreservation

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Publications

  1. An outlook into the novelties of bioprinting technology., The Pharma Innovation Journal 2021; SP-10(12): 01-0
  2. Cloning and expression of VP2 protein of infectious bursal disease virus. 2020. The Pharma Innovation Journal 2020; 9(6): 09-12
  3. Concurrent infection of feline infectious peritonitis and feline leukemia virus in a male cat,
  4. Dissolving microneedles in drug delivery: An outlook The Pharma Innovation Journal 2020; 9(5): 24-28
  5. Expression of immunogenic protein of infectious Bursal disease virus in mammalian vector pVAX.1.2021., The Pharma Innovation Journal 2021; SP-10(12): 1682-1687
  6. Methodologies for cloning and expression of immunodominant protein LipL32 of pathogenic Leptospira. The Pharma Innovation Journal 2020; 9(6): 09-12